Nick: Viola* Oggetto: Esperti d'Inglese, aiuto Data: 20/1/2009 14.27.55 Visite: 301
prima di sottoporre per la vintuordicesima volta questa sbobba al mio prof, e vedermela tirare appresso, e quindi evitare che io lo circoncida senza anestesia... qualche esperto di lingua inglese mi potrebbe dire se va bene? proprio la forma, i verbi etc tnx* Legionella pneumophila is widespread in aqueous environments and a common cause of nosocomial and community-acquired pneumonia. An accurate discrimination between L. pneumophila isolates is important in epidemiological investigations for identifying common-sources cases, routes of diffusion, and bacteria distribution in water networks [1,2]. The most commonly used techniques that allow for bacteria typing are based on the detection of genomic DNA polymorphisms [3]. A number of methods have been validated, including monoclonal antibody typing, plasmid analysis and multilocus enzyme electrophoresis [1,4]. More accurate techniques based on genomic DNA polymorphisms have been developed in the last decades, such as pulsed-field gel electrophoresis (PFGE), ribotyping, arbitrarily primed polymerase chain reaction (AP-PCR), restriction fragment length polymorphism analysis (RFLP), and more recently amplified fragment length polymorphisms (AFLP) [5,6,7,8]. Currently AFLP is the reference method for L. pneumophila Serogroup (Sg) 1 genotyping [8], although PFGE of DNA macrorestriction digests remains the most used due to its high discriminatory power [9,10]. PFGE protocol involves the DNA extraction from bacteria embedded in a gel, then the use of rare cutter restriction enzymes to generate a limited number (10 to 20) of high-molecular weights restriction fragments. "Cu chi tratti mpari e cu chi ferri gnuovi" Zanardi Traduzione: Se te la fai con persone degne puoi imparare qualcosa, se te la fai col ferraro puoi imparare solo a battere i chiodi. |
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