Nick: Viol4
Oggetto: x Black80
Data: 12/4/2003 15.50.56
Visite: 14
tiè, deliziati un pò anche tu, se poi ci tieni molto, un giro al microscopio te lo faccio fare...
Cellular adhesion molecules (CAMs) are a group of glicoproteins largely expressed on hematopoietic stem cells and involved in many processes like mobilization, homing and growth regulation. On a clinical ground, some authors correlated expression of particular subsets of CAMs (L-selectin, CD44, CD49d) on CD34+ stem cells with PMN and PLT engraftment after both PBSC and BM autologous transplantation. We analysed myeloid engraftment kinetics in 30 patients (16 NHL, 8 MM, 3 acute leukemia, 2 breast cancer and 1 multiple sclerosis). Median age was 49 years (range 19-59). Five received BM stem cells, 25 received PBSC. Three color flow cytometric assay was performed to quantify co-expression on CD34+ cells of a large panel of integrins (CD49b, CD49d, CD49f, CD11a, CD11b, CD11c, CD18) and L-selectin (tested by Lecam and Leu8). Each subset was quantified and correlated (Spearman's correlations) with myeloid recovery. Median number of CD34+ cells and CFU-GM infused was 5x106/Kg (range 1.5-26.3) and 16x104/Kg (range 1.4-94.5), respectively. All pts. showed a rapid and sustained engraftment of PMN >500/mm3 [median 10 days (range 8-17)] and PLT >20.000/mm3 [median 12 days (range 10-23)] and no late graft failure was observed. Total number of CD34+ cells strongly correlated with both PMN and PLT recovery [r -0.57 (p <.001) and -0.43 (p .018), respectively] but subsets as defined by the expression of different CAMs failed to improve the correlation with myeloid recovery. In particular, we were not able to find any correlation between PLT recovery and L-selectin expression as reported by other authors and by ourselves in a previous experience involving a smaller group of pts. In conclusion, acquisition of larger series of pts. is warranted to draw firm conclusions about mechanisms of stem cell homing in a clinical setting.
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x Black80 12/4/2003 15.50.56 (13 visite) Viol4
re:x Black80 12/4/2003 15.53.16 (20 visite) Black 80