Nick: MRosaria
Oggetto: re:Esperti d'Inglese, aiuto
Data: 20/1/2009 14.41.23
Visite: 92
In the last decades more accurate techniques based on genomic DNA polymorphisms have been developed, such as pulsed-field gel electrophoresis (PFGE), ribotyping, arbitrarily primed polymerase chain reaction (AP-PCR), restriction fragment length polymorphism analysis (RFLP), and more recently amplified fragment length polymorphisms (AFLP) [5,6,7,8].
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Currently AFLP is the reference method for L. pneumophila Serogroup (Sg) 1 genotyping [8], although PFGE of DNA macrorestriction digests remains the most used one due to its high discriminatory power [9,10]. PFGE protocol involves the DNA extraction from bacteria embedded in a gel, then the use of rare cutter restriction enzymes to generate a limited number (10 to 20) of high-molecular weight restriction fragments.
praticamente
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